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21.
Julio Flores-Gonzalez Juan Carlos Cancino-Díaz Leslie Chavez-Galan 《International journal of molecular sciences》2020,21(22)
Recent advances in the field of flow cytometry (FCM) have highlighted the importance of incorporating it as a basic analysis tool in laboratories. FCM not only allows the identification of cell subpopulations by detecting the expression of molecules in the cell membrane or cytoplasm, but it can also quantify and identify soluble molecules. The proper functioning of the FCM requires six fundamental systems, from those related to the transport of events to the systems dedicated to the analysis of information. In this review, we have identified the main considerations that every FCM user must know for an optimal antibody panel design, the quality systems that must govern the FCM protocols to guarantee reproducible results in research or clinical laboratories. Finally, we have introduced the current evidence that highlights the relevance of FCM in the investigation and clinical diagnosis of respiratory diseases, establishing important advances in the basic and clinical study of diseases as old as Tuberculosis along with the recent proposals for the monitoring and classification of patients infected with the new SARS-CoV2 virus. 相似文献
22.
Transient dynamics of tapping mode atomic force microscope (AFM) for critical dimension measurement are analyzed. A simplified nonlinear model of AFM is presented to describe the forced vibration of the micro cantilever-tip system with consideration of both contact and non-contact transient behavior for critical dimension measurement. The governing motion equations of the AFM cantilever system are derived from the developed model. Based on the established dynamic model, motion state of the AFM cantilever system is calculated utilizing the method of averaging with the form of slow flow equations. Further analytical solutions are obtained to reveal the effects of critical parameters on the system dynamic performance. In addition, features of dynamic response of tapping mode AFM in critical dimension measurement are studied, where the effects of equivalent contact stiffness, quality factor and resonance frequency of cantilever on the system dynamic behavior are investigated. Contact behavior between the tip and sample is also analyzed and the frequency drift in contact phase is further explored. Influence of the interaction between the tip and sample on the subsequent non-contact phase is studied with regard to different parameters. The dependence of the minimum amplitude of tip displacement and maximum phase difference on the equivalent contact stiffness, quality factor and resonance frequency are investigated. This study brings further insights into the dynamic characteristics of tapping mode AFM for critical dimension measurement, and thus provides guidelines for the high fidelity tapping mode AFM scanning. 相似文献
23.
This paper proposes an adaptive super-twisting decoupled terminal sliding mode control technique for a class of fourth-order systems. The adaptive-tuning law eliminates the requirement of the knowledge about the upper bounds of external perturbations. Using the proposed control procedure, the state variables of cart-pole system are converged to decoupled terminal sliding surfaces and their equilibrium points in the finite time. Moreover, via the super-twisting algorithm, the chattering phenomenon is avoided without affecting the control performance. The numerical results demonstrate the high stabilization accuracy and lower performance indices values of the suggested method over the other ones. The simulation results on the cart-pole system as well as experimental validations demonstrate that the proposed control technique exhibits a reasonable performance in comparison with the other methods. 相似文献
24.
Milinda Pattanayak Md Nadim F Hoque Zhaoyang Fan Ayrton A. Bernussi 《Science and Technology of Advanced Materials》2018,19(1):693-701
We report large amplitude modulation waveforms as large as ~ 10 V using vanadium dioxide micro-channel devices operating under current-controlled conditions. The self-sustained electrical oscillations were generated by controlling the applied current in the negative differential resistance region of the investigated devices. An appropriate value of internal capacitance was achieved as parasitic capacitance in the device structure to stabilize the electrical oscillations. This eliminates the need of an external pulsed source or any external passive component connected to the micro-channel devices. Amplitude and frequency of the oscillation were tuned by illuminating the device micro-channel with an external laser. An equivalent circuit model was developed to simulate the waveforms. A good agreement between experiment and simulation was verified. 相似文献
25.
与传统比例-积分-微分(PID)控制方法相比,滑模控制(SMC)方法可以比较容易地将不确定性纳入控制器设计中,从而增强系统的鲁棒性。探索了SMC技术在运载器主动段姿态控制中的工程应用,首先通过分析基于趋近律的SMC系统,提出了降低不连续切换项系数的需求,然后研究了基于干扰上界的SMC方法。三通道小偏差仿真结果验证了两种方法的控制效果,表明第2种控制器的鲁棒性更好,稳态误差小,同时发动机喷管摆角需求较小。 相似文献
26.
伴随着人类社会的向前推进,信息技术已经渗透到我们生活的方方面面,深刻地改变了教育事业,慕课就是在信息时代下诞生的一种新兴教学工具。但是,如何利用好这一工具却仍然是个值得研究的课题。本文从慕课的内涵出发,分析了将混合式教学模式应用于慕课这一教学平台的重要意义。并且,就混合式教学模式在慕课平台中的应用进行了分析。希望能够给相关工作者以一定的启发。 相似文献
27.
In this paper, a robust controller for a Six Degrees of Freedom (6 DOF) coaxial octorotor helicopter control is proposed in presence of actuator faults. Radial Base Function Neural Network (RBFNN), Fuzzy Logic Control approach (FLC) and Sliding Mode Control (SMC) technique are used to design a controller, named Fault Tolerant Control (FTC), for each subsystem of the octorotor helicopter. The proposed FTC scheme allows avoiding difficult modeling, attenuating the chattering effect of the SMC, reducing the rules number of the fuzzy controller, and guaranteeing the stability and the robustness of the system. The simulation results show that the proposed FTC can greatly alleviate the chattering effect, good tracking in presence of actuator faults. 相似文献
28.
为研究铝合金三角形波纹夹芯板对平头弹体的抗冲击性能及损伤特性,利用有限元软件ABAQUS/Explicit建立弹体冲击靶板的数值模拟模型,并结合实验验证了模型及其参数的有效性。基于数值计算结果,分析了三角形波纹夹芯板几何结构对其防护性能、失效模式和能量吸收的影响规律及机理,并与等面密度单层板进行对比分析,研究结果表明,靶体几何结构对其抗冲击性能存在影响,三角形波纹夹芯板抗冲击性能低于单层板抗冲击性能。此外,增加芯体拓扑结构夹角能显著提高三角形波纹夹芯板的抗冲击性能,并且靶板几何形状会对其失效模式及耗能特性存在影响。 相似文献
29.
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